After a file is locally approved because of its publisher’s reputation, removal of publisher approval at a later time does not remove local approval of that file.
Anything that removes approval from the publisher, including a change in reputation, a change in reputation approval settings, or completely disabling reputation approvals, only affects files that are encountered in the future.
If a publisher is no longer approved by reputation, its files can be returned to the unapproved state by manually removing the local approval on the Files on Computers or File Instance Details page for each instance. If a publisher is banned, that ban removes the approval from the file that was previously locally approved because of publisher reputation – it is not necessary to manually remove local approval for each instance.
Explicitly banning a file removes a local approval that occurred because of publisher approval.