You can remove the connection between VMware Horizon and a vCenter Server instance. When you do so, VMware Horizon no longer manages the virtual machines created in that vCenter Server instance.
Prerequisites
Delete all the virtual machines that are associated with the vCenter Server instance. For more information about deleting virtual machines, see "Delete a Desktop Pool" in the Setting Up Virtual Desktops in Horizon document.
Procedure
Results
VMware Horizon can no longer access the virtual machines created in the vCenter Server instance.